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To undo a reconciliation in QuickBooks Online, access the Chart of Accounts and locate the account containing the transaction. Open the transaction and unmark the R (Reconciled) status by clicking on it until it becomes blank. This removes the reconciliation for that specific transaction. If you need to undo an entire reconciliation, only an accountant with QuickBooks Online Accountant access can undo a full reconciliation from the Reconcile page. Ensure to verify your financial records before making adjustments to avoid discrepancies.